HPLC Products

With Challenging demands of scientist working in the field of Pharmaceuticals, Food Safety, Environmental, Forensic and Academics Bonna-Agela offers broad range of stationary phase chemistries to help customer to carry out rapid analysis, LC-MS Analysis, working through extreme pH conditions and extended temperatures. Bonna-Agela HPLC columns are manufactured by research and development scientists with innovative approach of playing with different ligands chemistries.

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Application notes:

A Mix-model HPLC Stationary Phase C18/SAX Improve Separation

Bonna-Agela Tech in USP- PQRI Column Database

Bonna-Agela Technologies Featured Applications

Shell Technology Solution for Faster Separations

Bonshell^® Columns are made up of shell particle to carry out faster separations with low back pressures. Bonshell^® has 0.5 μm porus shell covering solid core inside with diameter of 1.7 μm. With overall particle size of 2.7 μm. Bonshell^® Columns are ideal to be used with both UPLC and Conventional HPLC Systems which enables the scientist to easily transfer their existing method from HPLC to UPLC & vice-versa without changing the expensive hardware in HPLC system.

Venusil® HPLC columns are manufactured from the highest purity spherical silica particles on the market. The outstanding properties of the HPLC phases are results of subjecting high quality silica to Bonna-Agela’s innovative surface modificationand unique bonding processes. Our proprietary nanosurface modificationgenerates a very smooth and even surface. This reduces the interaction between the silica surface and polar compounds, resulting in symmetric peak shape even for very basic compounds. Based on this technology, a series of unique columns was developed to meet the tough requirements for the analysis of highly polar compounds. Lot-to-lot reproducibility is ensured by a stable bonding/end-capping process.

Bonna-Agela's Durashell series columns could be used under a wide pH range from 1.5 to 12.0. Durashell series columns have a hybrid technology stationary phase surface, followed by molecular modifications. The patented hybrid technology provides a strong hydrophobic protective layer on the silica surface, allowing the columns to be used at extremely high and low pH. It also reduces excessive hydrophobic interactions between the stationary phase and the analytes by reducing the bonding density, and yet maintains great interfacial kinetics for high efficiency. The stationary phases include C18(L), RP, C8 and NH2. Durashell C18(L) gives a good performance by decreasing the surface area to achieve the balance of the separation ability and the lifetime. Durashell RP column is the first-generation product designed for applications in a wide pH range from 1.5 to 11.0. A strong hydrophobic protective layer can be covered on the silica gel surface, which effectively prevents the destruction of the alkaline solution onto the silica. By using multi-functional bonding techniques, it shows better separation performance meanwhile.

C18 HPLC columns are used to analyze individual parts of chemical mixtures. Ion-exchange chromatography (SAX) is a process that allows the separation of ions and polar molecules based on their charge. We have designed a mix-mode HPLC column named as Optimix C18/SAX (2) made from 5 μm high purity spherical silica particles. A special silane with octadecyl chain embedded quaternary amine group is bonded to the silica particles.

Bezafibratum
European Pharmacopoeia 7.0, 1490-1491. Requirements: Resolution factor (R) for bezafibratum and its impurity should be > 5.0.

Cefradine
European Pharmacopoeia 7.0, 1620-1622. Requirements: Resolution factor (R) for cefradine and cefalexin should be > 4.

Clindamycin hydrochloride
European Pharmacopoeia 7.0: 1716-1717. Requirements: the retention time of clindamycin hydrochloride is around 10 min.

Pioglitazone Hydrochloride
USP34-NF29, 3933. Requirements: the resolution factor between pioglitazone hydrochloride and benzophenone should be >15, and the tailing factor for the two compounds should both be <1.5 respectively.